low melting-point agarose meaning in English
低熔点琼脂糖
Examples
- The results also explained that it was feasible that the genomic dna of bacillus subtilis were extracted and gained large dna fragments by low melting - point agarose embedding method
说明以低熔点琼脂糖包埋法提取枯草杆菌基因组dna ，并获得较大的dna片段，是完全可行的。 - Used the genomic dna extracted by low melting - point agarose embedding method as pcr template , the full length of structural genes of bacillus subtilis bio operon were gained by long pcr method
将该方法提取的基因组dna稀释100倍作为模板，采用长距离pcr方法，获得了枯草杆菌生物素操纵子基因全长。 - Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment , three methods were used to extract genomic dna of bacillus subtilis , i . e . low melting - point agarose embedding method , sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method . the genomic dna of bacillus subtilis were gained by these methods , and the operated programs of the methods were improved . the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods
大片段基因组dna的提取为了获得用于pcr扩增(长距离pcr扩增和分段pcr扩增)的高纯度、大片段(至少为pcr产物长度的4倍)的dna模板，应用三种方法：低熔点琼脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和细菌基因组dna提取试剂盒法，分别提取获得了枯草杆菌基因组dna ，并对3种方法的操作程序进行了不同程度的改进，结果表明：低熔点琼脂糖包埋法提取的基因组dna片段明显大于后两种方法，采用0 . 5琼脂糖凝胶电泳3h ，仍然跑不出加样孔。